NanoBRET Intracellular Target Engagement Assay

The NanoBRET assay provides real-time data of the binding affinity of a compound to kinase target including occupancy time  Intact cells provide a relevant environment for the compound-kinase interaction in regards of ion and ATP concentration, pH, or co-factors. In addition, to function inside the cell, compounds must overcome the cellular membrane.  Suitable for high-throughput screening Kinase activity testing in intact cells can be investigated with our Cellular Phosphorylation Assay

Setups: IC50 value determination in 10-dose duplicate assay format. Free choice for screening of the kinase targets of your choice. New kinase target development/validation is available upon request.  

Controls: We use DMSO as a negative control. In every project, testing of a standard reference compound is included in IC50 assay format as well.  

Turnaround time: 2-3 weeks. Expedited scheduling and data delivery can be arranged prior to study commencement.  Report: A detailed report including assay conditions and comprehensive evaluation of data as well as raw data for each analysis will be provided.  

Compound requirements: In brief, 50 to 100 µl of a 10 to 50 mM DMSO stock or 2-3 mg solid material is needed. 

Please refer to our FAQs for information regarding compound preparation and shipping.  

Please download our assay condition form.