The ORIS assay is well suited for the testing of compounds that inhibit undirected tumor cell migration. It is performed in a 96 well format and can be used for screening with medium throughput rates with high accuracy.
Assay procedure. (A) Tumor cells are seeded onto collagen I-coated multi-well plates equipped with a barrier that prevents cell adherence to the center of each well. After 18 hours, the barrier is removed (B) revealing a clear region in the center of the well. During a 24- to 48-hour incubation period in the presence of test compound (C), tumor cells migrate into this detection zone (D) which is monitored by fluorescent-labeled cells (E).
The assay is performed with the ORIS assay kit from Platypus.
The Wound Healing Assay (or Scratch Assay) measures the impact of compounds on undirected cell migration after wound-making via scratching the cell monolayer. The assay can investigate tumor and normal cells and is performed with Keyance system which enables real-time monitoring of cell migration.
Assay procedure. (A) Tumor cells grow to monolayers. (B) Using the WoundMaker, homogeneous scratches will be created mechanically with pins in a 96W format. (C) After the application of the test compound, plates incubate in the Keyance instrument for RT quantification of tumor cells migrating into the scratch zone (D).
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