Cell line authentication/
Mixed Sample Analysis
Promega GenePrint® 24 System
Mycoplasma testing: Using Lonza MycoAlert™ Assay
The MycoAlert™ Assay is a selective biochemical test that
exploits the activity of certain mycoplasmal enzymes.
The presence of these enzymes provides a
rapid screening procedure, allowing sensitive
detection of contaminating mycoplasma in a test sample.
The viable mycoplasma are lysed and the enzymes react with
the MycoAlert™ Substrate catalyzing the
conversion of ADP to ATP.
By measuring the level of ATP in a sample
both before and after the addition of the
MycoAlert™ Substrate, a ratio can be obtained which is indicative
of the presence or absence of mycoplasma
Sterility and viral testing:
We routinely test all cells that we receive and ship for bacteria and mold.
We also can perform viral and pathogen testing of tissue samples.
Available methods for performing viral testing of cell cultures include mouse, rat, and human viral panels using PCR-based methods.
We check the viability of all cell banks pre- and post-thaw.